Determination of Effective Dosage of Phyllanthus niruri to Modulate Stress in Tilapia, Oreochromis niloticus

Tol2-mediated transgenesis in tilapia (Oreochromis niloticus).

The Nile tilapia (Oreochromis niloticus) is an important species in aquaculture and an excellent model system for laboratory studies. Functional genetic analysis using this species has been difficult because existing methods for producing transgenics are inefficient. Here we show that the Tol2 transposon system can be used to create transgenic tilapia with high efficiency. We constructed a line...

Comparative transcriptome analysis of Nile tilapia (Oreochromis niloticus) in response to alkalinity stress.

To identify genes of potential importance to alkalinity tolerance, RNA sequencing (RNA-Seq) was performed to survey gill transcriptome profiles from freshwater (FW) and alkaline water (AW) exposed Nile tilapia (Oreochromis niloticus). A total of 22,724,036 (AW)/16,461,040 (FW) single-end reads were generated in which 20,304,348 (AW)/14,681,290 (FW) reads (90.0/89.72%) were aligned to the refere...

Growth and Stress Axis Responses to Dietary Cholesterol in Nile Tilapia (Oreochromis niloticus) in Brackish Water

Six isonitrogenous and isocaloric diets were formulated to contain 0% (control), 0.4, 0.8, 1.2, 1.6, or 2.4% dietary cholesterol and fed to juvenile Nile tilapia (Oreochromis niloticus) (2.20 ± 0.12 g) twice daily to apparent satiation for 8 weeks in triplicate at a salinity of 16. Fish fed 0.4% cholesterol showed a higher weight gain and specific growth rate and a lower feed coefficient ratio ...

ANALYSIS OF SEX DETERMINATION IN NILE TILAPIA (Oreochromis niloticus L.): A MOLECULAR GENETICS APPROACH

M. TARIQ EZAZ INSTITUTE OF AQUACULTURE PHD THESIS ii ABSTRACT Seven families of XX and YY homozygous Oreochromis niloticus were produced by mitotic gynogenesis from XY neofemales and their genetic status was verified by multilocus DNA fingerprinting and progeny testing. Two of these gynogenetic families and their corresponding diploid controls were used with 64 AFLP primer combinations in diffe...

The effect of feed ingredients on the settling velocity of feces in Tilapia (Oreochromis niloticus L.)